cacl2 heat shock method
CalciumChlorideimpartsapositivechargetothecellwall.SinceDNAisnegativelychargedmolecule,thepositivechargeonthecellwallimpartedbycalcium ...,Heatshockthecellsina42oCwaterbathfor45to60secondsandreturntoicefor1minute.Add1.2mlofLBbrothtothetube,mixand...
Calcium chloride transformation
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PlasmidDNAcanattachtoLPSbybeingaddedtothecellsolutiontogetherwithCaCl2.Thus,whenheatshockisapplied,thenegativelychargedDNA ...
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